| 貨號(hào) | 354248 | 規(guī)格 | 10ml |
|---|
| 供貨周期 | 現(xiàn)貨 | 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
|---|
BD全線(xiàn)特約代理，常用基質(zhì)等、備貨充足、價(jià)格優(yōu)勢(shì)大
BD產(chǎn)品貨號(hào)：354230354234356230356231356234356235356237
操作指南：
BD采用*利技術(shù)，從富含胞外基質(zhì)蛋白的EHS小鼠腫瘤中分離出BDMatrigel基底膜基質(zhì)，其主要成分由層粘連蛋白，Ⅳ型膠原，巢蛋白，硫酸肝素糖蛋白等組成，還包含生長(zhǎng)因子和基質(zhì)金屬蛋白酶等。BDMatrigel基底膜基質(zhì)在室溫條件下，聚合形成具有生物學(xué)活性的三維基質(zhì)，模擬體內(nèi)細(xì)胞基底膜的結(jié)構(gòu)、組成、物理特性和功能，有利于體外細(xì)胞的培養(yǎng)和分化，以及對(duì)細(xì)胞形態(tài)、生化功能、遷移、侵染和基因表達(dá)的研究。
BDMatrigel基底膜基質(zhì)形成的三維培養(yǎng)基質(zhì)，可促進(jìn)上皮細(xì)胞、肝細(xì)胞、Sertoli細(xì)胞、黑色素瘤細(xì)胞、血管內(nèi)皮細(xì)胞、甲狀腺細(xì)胞及毛囊細(xì)胞等的貼壁與分化。同時(shí)，Matrigel還能影響乳腺上皮細(xì)胞的蛋白表達(dá)，支持外周神經(jīng)的新生和牛輸卵管上皮細(xì)胞的分化。高濃度的Matrigel適用于研究體內(nèi)血管生成和腫瘤細(xì)胞遷移及腫瘤模型的建立等。
BD基質(zhì)膠/基質(zhì)膜cornning354248354230特性：
Matrigel基質(zhì)會(huì)有色差變化（淡黃色到深紅色），是由于酚紅和碳酸氫鹽與CO2作用引起的，但是與5％CO2平衡后色差即會(huì)減少。凍融后，輕輕搖晃試劑瓶使Matrigel分散均勻。所有操作均需在無(wú)菌環(huán)境下進(jìn)行，試劑瓶瓶蓋可用70％乙醇擦拭，并自然干燥。應(yīng)使用預(yù)冷的移液器以保證Matrigel呈勻漿狀。
細(xì)胞可在0.5mm厚度的Matrigel基質(zhì)層表面生長(zhǎng)，也可在1mm厚度的Matrigel三維基質(zhì)內(nèi)生長(zhǎng)。過(guò)度稀釋的Matrigel會(huì)形成非膠質(zhì)的蛋白層，可以用于細(xì)胞貼壁，但不能用于細(xì)胞的分化研究。
注意：可將凍融后的Matrigel分裝在多個(gè)小管，所有分裝均需用預(yù)冷的凍存管，迅速冷凍并保存，避免多次凍融。
Matrigel在22-35℃溫度環(huán)境下快速成膠，因此溶解時(shí)在4℃冰上過(guò)夜凍融（4度時(shí)會(huì)隨著溫度的上升部分成膠）。所有用品在使用前需置于冰浴，必須使用預(yù)冷的移液管、吸頭及小管操作Matrigel。成膠后的Matrigel可以在4℃24－48小時(shí)后重新呈液態(tài)。
推薦包被與成膠方法：
注意：為了保證Matrigel基質(zhì)膜的成膠性能與穩(wěn)定性，稀釋濃度不應(yīng)低于1：3，可用無(wú)血清培養(yǎng)基稀釋?zhuān)琈atrigel成膠后立即使用。
薄膠成膠方法：
1.凍融后，用預(yù)冷的移液槍頭混勻Matrigel基質(zhì)成勻漿狀。
2.將需要使用的培養(yǎng)板置于冰上，加入濃度為50μL/cm2生長(zhǎng)面積的Matrigel基質(zhì)。
3.在37℃放置30分鐘，即可使用。
厚膠成膠方法：
1.凍融后，用預(yù)冷的移液槍頭混勻Matrigel基質(zhì)成勻漿狀。
2.將需要使用的培養(yǎng)板置于冰浴，將培養(yǎng)的細(xì)胞與Matrigel基質(zhì)混合，用移液槍頭使其懸浮于基質(zhì)中。加入濃度為150－200μL/cm2生長(zhǎng)面積的Matrigel基質(zhì)。
3.在37℃放置30分鐘，可成膠?？梢约尤爰?xì)胞培養(yǎng)的基質(zhì)，也可使細(xì)胞直接生長(zhǎng)在膠表面。
薄層包被方法：
1.凍融后，用預(yù)冷的移液槍頭混勻Matrigel基質(zhì)成勻漿狀。
2.根據(jù)使用需要，采用無(wú)血清培養(yǎng)基稀釋Matrigel基質(zhì)。根據(jù)實(shí)驗(yàn)需要確定*包被濃度。
3.將稀釋的Matrigel基質(zhì)包被于所需的培養(yǎng)器皿中，包被量至少覆蓋整個(gè)器皿的生長(zhǎng)表面。室溫下孵育1小時(shí)。
4.去除未結(jié)合的Matrigel，用無(wú)血清培養(yǎng)基輕輕地沖洗。
本公司提供BD基質(zhì)膠/基質(zhì)膜cornning354248354230，如有需要?dú)g迎來(lái)咨詢(xún)！
351130Falcon96WellMultiwellInsertSystemwith1.0µmPoreTranslucentPolyester(PET)Membrane,withFeederTrayandLid,1/Case
351131Falcon96WellMultiwellInsertSystemwith1.0µmPoreTranslucentPolyester(PET)Membrane,withFeederTrayandLid,5/Case
351155CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs
351156CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs
351157CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs
351158CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs
351161CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs
351162CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs
351163CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs
351164CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs
351181Falcon24WellMultiwellInsertSystemwith1.0µmPoreHighDensityPolyester(PET)Membrane,5/Case
351183FalconHTS24WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pack,5/Case
351184FalconHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pack,1/Case
351185FalconHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pack,5/Case
351186Falcon24WellPolystyreneFeederTray,withLid,Sterile,5/Pack,5/Case
353015CorningGentestPre-coatedPAMPAPlateSystem,withLid,5/Case
353137CorningCELLineDisposableBioreactor
353924Falcon96WellFeederPolystyreneTray,withLid,Sterile,5/Pack,5/Case
353925Falcon®96SquareWellAngledBottomNotTreatedMultiwellInsertSystem,withLid,Sterile,5/Case
353928Falcon96SquareWellFlatBottomMicroplate,withLid,Sterile,5/Pack,5/Case
353938Falcon96SquareWellAngledBottomInsertPlate,withLid,Sterile,5/Case
354001Corning®EpidermalGrowthFactor(EGF),MouseNatural(CultureGrade),100µg,1/Pack
354008Corning®Fibronectin,Human,1mg
354009Corning®7SNerveGrowthFactors(NGF),MouseNatural,100µg,1/Pack
354010Corning®EpidermalGrowthFactor(EGF),MouseNatural(ReceptorGrade),100µg,1/Pack
354022CorningBioCoatPoly-D-Lysine1536WellBlackFlatBottomMicroplate,5/Case
354037Corning®Insulin-likeGrowthFactor(IGF-I),HumanRecombinant(CultureGrade),10µg,1/Pack
354039Corning®TransformingGrowthFactor-b(TGF-b),HumanNatural,1µg,1/Pack
354043Corning®Interleukin-2(IL-2),HumanRecombinant,10,000BRMPUnits,1/Pack
354048Corning®Granulocyte-MacrophageColonyStimulatingFactors(GM-CSF),HumanRecombinant,1µg,1/Pack
354051Corning®Platelet-DerivedGrowthFactor-BB(PDGF-BB),HumanRecombinant,10µg,1/Pack
354052Corning®EpidermalGrowthFactor(EGF),HumanRecombinant,100µg,1/Pack
354058Corning®Interleukin-3(IL-3),MouseRecombinant,10µg,1/Pack
354060Corning®BasicFibroblastGrowthFactors(bFGF),HumanRecombinant,10µg,1/Pack
354066Corning®TumorNecrosisFactor-a(TNF-a),HumanRecombinant,10µg,1/Pack
354068Corning®Interleukin-4(IL-4),HumanRecombinant,5µg,1/Pack
354077Corning®BioCoat™Poly-D-Lysine35mmCoverslipBottomTC-TreatedDishes,20/Pack,20/Case,Nonsterile
354085Corning®BioCoat™Poly-L-Lysine12mm#1GermanGlassCoverslip,40/Pack,80/Case
354086Corning®Biocoat™Poly-D-Lysine12mm#1GermanGlassCoverslip,BulkPacked,80/Case
354087Corning®BioCoat™Poly-D-Lysine/Laminin12mm#1GermanGlassCoverslip,BulkPacked,80/Case
354088Corning®BioCoat™Fibronectin22mmRound#1GermanGlassCoverslip,BulkPacked,60/Case
354089Corning®BioCoat™CollagenI22mmRound#1GermanGlassCoverslip,BulkPacked,60/Case
354105Corning®StemCellFactor(SCF),HumanRecombinant,10µg,1/Pack
354107Corning®VascularEndothelialGrowthFactor(VEGF),HumanRecombinant,10µg,1/Pack
354115Corning®T-CellCultureSupplementwithConA(IL-2CultureSupplement),Rat,100mL,1/Pack
354141CorningBioCoatHTS24WellMultiwellAngiogenesisSystemforEndothelialCellInvasion,1/Pack,1/Case
354142CorningBioCoatHTS24WellMultiwellAngiogenesisSystemforEndothelialCellInvasion,1/Pack,5/Case
354143CorningBioCoatBioCoatFibronectinHTS24WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Case
354144CorningBioCoatFibronectinHTS24WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Pack,5/Case
354147CorningBioCoatFibronectinHTS96WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Pack,1/Case
354148CorningBioCoatFibronectinHTS96WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Pack,5/Case
354149CorningBioCoatAngiogenesisSystem:EndothelialCellTubeFormation,96WellBlackMultiwellPlate,1/Case
354150CorningBioCoatAngiogenesisSystem:EndothelialCellTubeFormation,96WellBlackMultiwellPlate,5/Case
354151CorningHumanUmbilicalVeinEndothelialCells,HUVEC-2Cells(>5x105Cells),1Cryovial/Pack
354165CorningBioCoatMatrigelTumorInvasionFluoroBlok24WellPlate,withLid,1/Pack,1/Case
354166CorningBioCoatMatrigelTumorInvasionFluoroBlok24WellPlate,withLid,1/Pack,5/Case
354167CorningBioCoatMatrigelTumorInvasionFluoroBlok96WellPlate,withLid,1/Pack,1/Case
354168CorningBioCoatMatrigelTumorInvasionFluoroBlok96WellPlate,withLid,1/Pack,5/Case
354210Corning®Poly-D-Lysine,20mg
354216CorningCalceinAMFluorescentDye,500µg,10x50µg
354217CorningCalceinAMFluorescentDye,1mg
354218CorningDiIC12(3)FluorescentDye,100mg
354221Corning®rLaminin-521,Human,100µgVial
354222Corning®rLaminin-521,Human,1mg(10x100µgVials)
354223Corning®rLaminin-521,Human,5mg(50x100µgVials)
354230Corning®Matrigel®GrowthFactorReduced(GFR)BasementMembraneMatrix,*LDEV-Free,10mL
354232Corning®Laminin,Mouse,1mg
354233Corning®CollagenIV,Mouse,1mg
354234Corning®Matrigel®BasementMembraneMatrix,*LDEV-Free,10mL
354235Corning®Dispase,100mL
354236Corning®CollagenI,RatTail,100mg
產(chǎn)品型號(hào)：356230 356231 356234
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